Original Research Article
Year: 2017 | Month: March | Volume: 7 | Issue: 3 | Pages: 126-134
Expression Analysis of p53 and Cyclooxygenase-2 in Oral Lichen Planus
Zafar Iqbal Bhat1,3, Akanksha Marwah1, Md.Zafaryab3, Nita Khurana2, Aadithya B Urs1, Mahesh Verma1, Vijay Garg2, A.K. Agarwal2, Sunita Gupta1, M.M.A Rizvi3
1Maulana Azad Institute of Dental Sciences, 2Maulana Azad Medical College & LNJP Hospital,
3Genome Biology Lab. Department of Biosciences, Jamia Millia Islamia,
Department of Oral Medicine & Radiology, Maulana Azad Institute of Dental Sciences, New Delhi.
Corresponding Author: Zafar Iqbal Bhat
Background: Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin. WHO classifies OLP as a potentially malignant disorder; however, the underlying mechanisms initiating development of cancer in OLP lesions have not been understood. The p53 tumor suppressor plays an important role in many tumors and an increased expression of p53 protein has been seen in OLP lesions. Cyclooxygenase-2 (COX-2) is a key enzyme for inflammatory processes and cellular proliferation, which has also been observed to over express in this chronic inflammatory condition. The aim of this study was to find the expression of p53 and COX2 in histopathologically diagnosed specimens of OLP and to explore the possible correlation between them.
Method: This study was conducted on 60 histopathologically diagnosed cases of OLP and 10 normal oral mucosa specimens as controls. Immunohistochemistry was performed to find out the p53 and COX -2 protein expression.
Result: The expression of p53 was positive in 80% of the cases of OLP as compared to normal oral mucosa, however, COX-2 expression was positive in 83.34%. No significant correlation was found between p53 and COX-2.
Conclusion: p53 and COX-2 have shown significant increase in expression in OLP cases as compared to normal, therefore these two markers can be used for routine histopathological examination to identify OLP lesions for development of OSCC.
Key words: COX2, Oral Lichen Planus, p53