Original Research Article
Year: 2019 | Month: September | Volume: 9 | Issue: 9 | Pages: 15-18
In-Vitro Activity of Doripenem vs. Imipenem & Meropenem against Clinical Isolates of Pseudomonas and Other Oxidase-Positive Gram-Negative Bacilli
Priya Singh1, Richa Misra2
1Department of Microbiology, Swargiya Dadasaheb Kalmegh Smruti Dental College & Hospital, Nagpur, India
2Department of Microbiology, Division Bacteriology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, India
Corresponding Author: Richa Misra
ABSTRACT
Background & objectives:Doripenem is approved by United States Food and Drug Administration(US FDA) for the treatment of complicated urinary tract infections (cUTI), complicated intra-abdominal infections (cIAI) and in Europe for ventilator-associated pneumonia (VAP).We attempted to compare the in vitro activity of doripenem with imipenem and meropenem using current CLSI guidelines among clinical isolates of Pseudomonas spp. and other oxidase positive Gram-negative bacilli from clinical samples.
Methods:Eighty consecutive clinical isolates were included in the study. Samples included were pus (n=29), blood (n=7), urine (n=24) and 20 sputum (n=20). MIC values of the imipenem, meropenem and doripenem were determined by E tests. Out of 72 Polymyxin B sensitive isolates 34 were Pseudomonas aeruginosa, 30 Fluorescent group and 8 Alcaligenes group. Polymyxin B (n=8) resistant isolates were identified as Burkholderia group. Pseudomonas aeruginosa ATCC 29853 was used as quality control strain.
Results:In the Polymyxin B sensitive group (n=72), 29.1%, 23.6% and 26.3% isolates were susceptible to imipenem, meropenem and doripenem respectively. In the Polymyxin B resistant group (n=8) only one isolate was sensitive to imipenem and meropenem while 5 were moderately sensitive to doripenem.
Interpretation & conclusions:Among Polymyxin B sensitive isolates meropenem was found least susceptible as compared to doripenem and imipenem. Doripenem may be a valuable alternative in Polymyxin B resistant isolates for the treatment of serious infections and in the intensive care unit where patients have predisposing conditions for seizures.
Key words: Doripenem, meropenem, imipenem, E test, Pseudomonas, Non-fermenting gram negative bacilli