Original Research Article
Year: 2019 | Month: July | Volume: 9 | Issue: 7 | Pages: 30-36
Spectrum of Microorganisms Found in Chronic Suppurative Otitis Media & Their in-Vitro Antimicrobial Sensitivity Pattern
Deepesh Aggarwal1, Anita Pandey2, Sonal Jindal3, Anshul Bansal4
1M.Sc. student, 2Professor and Head, 3Assistant Professor, Post Graduate Department of Microbiology, 4Assistant Professor, Department of Otorhinolaryngology
Subharti Medical College and Associated Chattrapati Shivaji Subharti Hospital Meerut (UP) India
Corresponding Author: Anita Pandey
ABSTRACT
Background: Chronic Suppurative Otitis Media (CSOM) is common health problem in developing countries and has potential to cause severe damage to patients from mild pus discharge to permanent hearing loss. Knowledge of spectrum of microorganisms and their antimicrobial sensitivity pattern is essential so that early and effective therapeutic measure can be taken for better outcome.
Objective: The study was carried out to know the spectrum of microorganisms found in CSOM and their antimicrobial sensitivity pattern.
Materials and Methods: A total of 103 ear discharges collected from clinically suspected cases of CSOM were subjected for isolation and identification of bacterial and fungal pathogen using standard bacteriological and mycological methods respectively. Antimicrobial sensitivity testing of bacterial isolated was performed by Kirby-Bauer’s disk diffusion method as per the Clinical Laboratory Standards Institute (CLSI) guidelines.
Result: A total of 86.41% cases were culture positive. Pure bacterial pathogen was isolated from 86.52% followed by pure fungal pathogen 7.87% and mixed pathogen (bacteria and fungus) from 5.62% cases. Pseudomonas aeruginosa and Staphylococcus aureus were the predominant bacterial pathogens and Aspergillus species were the predominant fungal pathogen isolated. MRSA and ESBL producers were 60% and 55.56% respectively.
Conclusion: Resistant microorganisms were isolated from cases of CSOM in our hospital. Therefore, such study may help as a baseline data to start empirical therapy while waiting for culture reports.
Key words: Chronic suppurative otitis media, bacteria, fungus, sensitivity tests