Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 81-88
Evaluation of Phytochemical and Antimicrobial Potential of Endophytic Fungi Nigrospora Oryzae Isolated from Terminalia Bellerica Roxb.
Jagadevi Shivaputrappa1, Vidyasagar GM2
1Research Scholar, 2Professor, Medicinal Plants and Microbiology Research Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, Kalaburagi- 585106, Karnataka, India.
Corresponding Author: Vidyasagar GM
ABSTRACT
Microorganisms are divided in to two categories; they are beneficial and harmful microorganisms. Beneficial microorganisms helps human beings where as harmful microorganisms causes several dreadful diseases to human beings. There are several antimicrobial drugs are available but the microbes became resistant to available drugs. So, there is an urgent need to develop a new therapeutic agent with least side effects. Terminalia bellerica is an important ethnomedicinal plant and this is the first report of endophytic fungi from this plant.
Objective: To evaluate the phytochemical and antimicrobial activity of an endophytic fungi Nigrospora oryzae.
Materials and methods: The phytochemical analysis of all the extracts of fungi was carried out by referring the standard procedures and antimicrobial activity was carried out by agar well diffusion method.
Results: The phytochemical investigation of all the extracts of fungi showed the presence of alkaloids, phenols, flavonoids, tannins, saponins, steroids, triterpenes and glycosides. The methanol, ethanol and ethyl acetate extracts of endophytic fungi were tested against five human pathogenic Bacteria like, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli and four Candida species like, C. albicans [MTCC 1637], C. glabrata [MTCC 3019], C. haemulonii [MTCC 1966] and C. tropicalis [MTCC 230] by agar well diffusion method. The methanolic and alcoholic extract of N. oryzae at 40mg/ml showed maximum activity against S. aureus (17mm), S. typhi (18mm) , C. Tropicalis (14mm) and C. albicans (15mm), respectively.
Conclusion: N. oryzae possess several bioactive molecules which are responsible for antimicrobial potential, may serve as an alternative source for the treatment and control of several microbial infections.
Key words: Terminalia bellerica, Nigrospora oryzae, Stem bark, endophytic fungi.