Review Article
Year: 2018 | Month: June | Volume: 8 | Issue: 6 | Pages: 344-351
Sputum as a Diagnostic Matrix for Respiratory Disease Screening
Subasa Chandra Bishwal1, Ranjan Kumar Nanda2, Rajendra Kumar Behera3
1Research Fellow, Translational Health Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India and School of Life Sciences, Sambalpur University, Jyoti Vihar, Sambalpur, Odisha, India.
2Group Leader, Translational Health Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi.
3Associate Professor, School of Life Sciences, Sambalpur University, Jyoti Vihar, Sambalpur, Odisha, India.
Corresponding Author: Rajendra Kumar Behera
ABSTRACT
Mucus hyper-secretion is a common feature in most of respiratory diseases. Sputum contains white blood cells, cellular debris, dead tissue, serous fluid and offers an easy, inexpensive and non-invasive method of disease diagnosis. Sputum is rich in lipids, glycoconjugates and proteins. These molecules could be secreted from respiratory tract, lungs and infected sites as well as of pathogen origin. Complete sputum proteome profiling could be useful by adopting mass spectrometry methods to discover suitable clinical markers that could explain the disease and could be used for development of easy to use cost effective diagnostic test. The advancement of proteomics tools will be useful to carry out such biomarker discovery work. In this article, we will be discussing about the sputum proteome analysis in different respiratory disease conditions. Successful application of proteomics tool for understanding perturbed pulmonary disease conditions using sputum as a diagnostic matrix will be discussed.
Key words: Diagnosis, Noninvasive, Proteomics, Respiratory, Sputum.