Original Research Article
Year: 2018 | Month: April | Volume: 8 | Issue: 4 | Pages: 63-70
Implications of Vitamin D Receptor Gene FokI and BsmI Polymorphisms in Type 2 Diabetes among Saudi Population
Fathy Mohamed Elfasakhany1, Abdulah Mousa Karawagh2, Shahid Saied Siddiqui3
1Associate professor of Medical Biochemistry and Molecular Biology, Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia; Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
2Consultant of Internal Medicine, Department of Internal Medicine, King Abdulla Medical Complex, Jeddah, Saudi Arabia.
3Professor of Medical Genetics, Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia.
Corresponding Author: Fathy Mohamed Elfasakhany
ABSTRACT
Background: The role of vitamin D receptor (VDR) has been involved in different disease states such as cancer, bone disease, tuberculosis and metabolic syndrome. Research on association between VDR polymorphisms and type 2 diabetes mellitus (T2DM) in various ethnic populations and different environmental conditions like atmospheric temperature are so far uncertain. Vitamin D is assumed to function as transcription regulator in controlling the β-cell insulin secretion by its association with its receptor complex.
Aim: This study analyzed the relationship between VDR FokI and BsmI polymorphisms and the susceptibility toT2DM among Saudi subjects in Makkah region that has a warm to hot climate through the whole year.
Materials and Methods: Genomic DNA was extracted from the peripheral blood leucocytes and genotyped for the single nucleotide polymorphisms (SNPs) of FokI (T/C) and BsmI (A/G) using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP).
Results: Our results demonstrated that in Makkah region, which has a hot atmosphere consistently, there is no significant difference in the genotype and allele frequencies of VDR FokI and BsmI polymorphisms between the subjects withT2DM and the control group (p> 0.05). Thus our results validate and affirm various research studies already done in different ethnic populations (European, Chinese, and Tunisian subjects) and geographical areas.
Conclusion: The VDR FokI and BsmI polymorphisms may not be related to the vulnerability to T2DM among Saudi population in Makkah area.
Key words: Type 2 diabetes mellitus, Vitamin D Receptor, Gene Polymorphism, restriction endonuclease, polymerase chain reaction.