Original Research Article
Year: 2017 | Month: Aug | Volume: 7 | Issue: 8 | Pages: 342-350
Synthesis of Silver Nanoparticle from Saraca Asoca Leaf Extract & Study of Its Antibacterial and Antioxidant Activity
Lakshya Dharamdasani1, Saman Pathan2, Prerna Bodhankar3, Kriti Rai1, Pallavi Nair1
1Pinnacle Biomedical Research Institute, Bhopal-462003, India
2Research Associate, Pinnacle Biomedical Research Institute, Bhopal
3Scholars, Institute for Excellence in Higher Education
Corresponding Author: Lakshya Dharamdasani
ABSTRACT
The field of biotechnology has crossed yet another milestone with development of advanced stream as Nanotechnology. With huge potential of nanotechnology the human race can make immense profit and benefits in his life. One of the most performed uses of nanotechnology comes with the synthesis of silver nanoparticles that is currently a topic of research. These silver nanoparticles or AgNPs have been synthesized from variety of different plant species and have been reduced for detection of antimicrobial activities of various parts of plants. Apart from being eco-friendly, in our present study silver nanoparticles were being generated from the leaves of Saraca asoca to evaluate antibacterial effects of plant. The bactericidal effect of biosynthesized nanoparticles was tested against strains of Bacillus subtilis and Proteus vulgaris. The antibacterial effect was determined using well diffusion method. Both the extract and nanoparticles showed antibacterial activity ranging between various concentrations. Further characterization of silver nanoparticles was done using UV-Vis absorption spectroscopy ranging between 250-800nm. The particle size of the synthesized AgNPs was determined with the help of particle size analyzer. The anti-oxidant activity of the extract was also determined where methods employed included DPPH assay and Reducing Power assay. Lastly, based on the results obtained it was concluded that the plant variety used was effective in bio synthesis of silver nanoparticles and as such can be successively used in various other fields of biotechnology and nanotechnology.
Key words: Bacillus subtilis, DPPH assay, Proteus Vulgaris, reducing power assay, Silver nanoparticle and Well diffusion assay