Original Research Article
Year: 2017 | Month: Aug | Volume: 7 | Issue: 8 | Pages: 155-164
Synthesis of Silver Nanoparticle from Spathodea Campanulata Leaf Extract and Study of Its Antimicrobial and Antioxidant Activity
Kriti Rai1, Saman Pathan2, Lakshya Dharamdasani1, Pallavi Nair1, Prerna Bodhankar3
1Pinnacle Biomedical Research Institute, Bhopal-462003, India
2Research Associate, Pinnacle Biomedical Research Institute, Bhopal
3Institue for Excellence in Higher Education, Scholars
Corresponding Author: Kriti Rai
ABSTRACT
The developments and progress in artificial intelligence and molecular technology have spawned a new form of technology; Nanotechnology. Nanotechnology could give the human race eternal life. Eco friendly methods of green mediated synthesis of nanoparticle are the current research in the appendage of nanotechnology. Silver nanoparticle (AgNPs) have been synthesized from myriad of plant species and used to determine the antimicrobial activity of various micro-organisms. In the present study silver nanoparticle were synthesized from leaf extract of Spathodea campanulata. The formation of the silver nanoparticle was monitored using UV-Vis absorption spectroscopy in the range from 250-800 nm. The particle size and range was further confirmed using particle size analyzer. The antibacterial activity of extract and nanoparticle was determined using well diffusion method. The bacterial species taken into consideration were gram positive- Bacillus cereus and Actinomyces and both extract and silver nanoparticle showed antibacterial activity at concentration ranging between 25, 50, 75, 100 mg/ml. The extract also showed anti-oxidant activity, the method employed were DPPH assay and reducing power assay. Based on the result obtained, it can be concluded that the plant resources can efficiently used in the production of silver nanoparticle and it could be utilized in various fields such as biomedical, nanotechnology, microbiology etc.
Key words:Silver nanoparticle, well diffusion assay, DPPH assay, reducing power assay