Original Research Article
Year: 2017 | Month: February | Volume: 7 | Issue: 2 | Pages: 281-290
Genetic Polymorphism of DNA Repair Genes (XRCC1, XRCC2 & XRCC3) in Breast Cancer Patients from Rural Maharashtra
Kailas D. Datkhile1, Suresh J. Bhosale2, Madhavi N. Patil1, Tejasvi S. Khamkar1, Pratik P. Durgawale1, Satish V. Kakade3
1Molecular & Genetic Laboratory, 2Department of Surgery, 3Department of Preventive and Social Medicine,
Krishna Institute of Medical Sciences University, Taluka-Karad, Dist-Satara, Pin-415 110 Maharashtra, India
Corresponding Author: Kailas D. Datkhile
ABSTRACT
Background & Objectives: Breast cancer is a major concern of health risk, moreover the leading cause of cancer causing deaths in women of rural parts of India. In this study, we focused to determine the frequency of polymorphisms in DNA repair genes, XRCC1 at codon (cd) 194, cd 280, cd 399, XRCC 2 at cd 188 and XRCC3 at cd 241 to evaluate their role in breast cancer risk.
Methods: We used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to analyze XRCCgenes polymorphisms in 150 breast cancer women and 200 age matched disease-free controls.
Results: The result from our study showed that allele frequencies of selected genes were not statistically different between the groups for XRCC1 Trp194, Gln399, XRCC2 His188 and XRCC3 Met241. XRCC1 His280 (OR= 4.14; 95% CI= (2.63-6.53); p= <0.0001) genotype significantly increased the risk of breast cancer.
Interpretation & conclusions: This study indicates that polymorphisms in cd280 of XRCC1 gene could play a role in modifying genetic susceptibility of individuals towards breast cancer among women from rural Maharashtra. It is apparent from our findings that larger part of the female had age at first delivery lower than 20 years which could be the probable risk factor for development of breast cancer. Thus, the case-control study suggest that selected DNA repair genes represent genetic determinants in breast carcinogenesis along with other risk factors in the rural Indian population.
Key words: Genetic polymorphisms, PCR-RFLP, XRCC1, XRCC2, XRCC3