Original Research Article
Year: 2016 | Month: December | Volume: 6 | Issue: 12 | Pages: 184-190
Evaluation of Microscopy and Polymerase Chain Reaction Methods for Identification of Trypanosoma Vivax in Cattle from Three Selected Abattoirs in Kaduna State, Nigeria
Muhammad A.A1, Sambo F2, Danladi L.O3, Salawu M.J1, Jarmai K.Y1, Abubakar S1, Abubakar A.T1, Musa A.M1, Jega Z.H4, Mohammad B5
1Trypanosomiasis Research Department, Nigerian Institute for Trypanosomiasis Research, No. 1 Surame Road, U/Rimi, P. M. B. 2077, Kaduna, Nigeria.
2Vector and Parasitology Departments, Nigerian Institute for Trypanosomiasis, Research, No. 1 Surame Road, U/Rimi, P. M. B. 2077, Kaduna, Nigeria.
3Research Planning, Monitoring, Extension and Statistics Department, Nigerian Institute for Trypanosomiasis, Research, No. 1 Surame Road, U/Rimi, P. M. B. 2077, Kaduna, Nigeria.
4Epidemiology Unit, Nigerian Institute for Trypanosomiasis, Research, Suleja, Niger State, Nigeria.
5Extension Services Unit, Consultancy and Extension Services Division, Nigerian Institute for Trypanosomiasis, Research, No. 1 Surame Road, U/Rimi, P. M. B. 2077, Kaduna, Nigeria.
Corresponding Author: Muhammad A.A
ABSTRACT
Microscopy and Polymerase Chain Reaction technique was used to evaluate 150 blood samples from cattle in three abattoirs in Kaduna State to detect the presence of Trypanosoma vivax. Out of the 150 blood samples collected and tested for the presence of T. vivax using Microscopy,13 samples from Tudun-wada, Kawo and Makera abattoirs were found to positive giving a total prevalence of 26.0%. Tudun-wada abattoir had the highest prevalence with 16.0% while 3.0% and 2.0% for Kawo and Makera abattoirs respectively. Tudun- wada abattoir at 0.016 was seen to be significantly different at p<0.05 from the remaining two abattoirs with 0.950 and 0.934. Thereafter, these samples were further subjected to PCR to detect the presence of this parasite, only 3 bands were confirmed to contain DNA for T.vivax as seen in Plate II after agarose gel electrophoresis at 150 bp and Plate I showing no band at 150bp.Thus, indicating false positive result from 13 samples obtained from Microscopy in this study. It was then concluded that Microscopy which is the easiest technique for parasitic detection is not a gold standard for detection of T. vivax but PCR which is two to three fold more sensitive and specific.
Key words: Abattoir, DNA, Microscopy, PCR, Primer, T. vivax.