Original Research Article
Year: 2016 | Month: November | Volume: 6 | Issue: 11 | Pages: 118-131
Cytoprotective, Antihyperglycemic and Antioxidative Effect of Naringenin on Liver and Kidneys of Swiss Diabetic Mice
Anupama Sharma1, Abani K Patar1, Surya Bhan2
1Research Scholar, 2Assistant Professor,
Department of Biochemistry, North-Eastern Hill University, Shillong, Megahalaya-793022.
Corresponding Author: Surya Bhan
ABSTRACT
Long-term effects of diabetes include serious health complications of liver and kidneys such as diabetic hepatopathy, a glycogen load on hepatocytes, and nephropathy, the damage to the glomerular capillaries. Polyphenols such as flavonoids have shown to possess effective antioxidant properties. In this study, the effect of naringeninas an antitoxic, antioxidative, and antihyperglycemicagent was studied and compared with standard L-ascorbic acid and metformin in diabetic Swiss albino mice. Acute toxicity, blood glucose, glycosylated hemoglobin, serum hepatic marker enzymes, antioxidant enzyme status both in vitro and in vivo, lipid peroxidation, histological examination, and transmission electron microscopy studies were conducted in streptozotocin-induced diabetic mice. Diabetes was induced by intraperitoneal injections with streptozotocin. Significantly higher levels of blood glucose and glycosylated hemoglobin; elevated levels of serum hepatic marker enzymes; lower levels of the antioxidative enzymes; and raised levels of malondialdehyde were seen. Hyperglycemia also affected the liver and kidney tissues, as seen under both light and transmission electron microscope in diabetic mice. Administration of intraperitoneal injections of naringenin to the diabetic mice for a period of 28 days prevented oxidative stress and lowered blood glucose and glycosylated hemoglobin including the serum hepatic marker enzymes and lipid peroxidation. Acute toxicity studies revealed naringenin to be less toxic. Further, histopathological and electron microscopy studies revealed the antioxidative nature of naringenin. These results suggested the positive effects of naringenin in mice, proving its further use in the management of diabetic hepatopathy and nephropathy.
Key words: Diabetes, Naringenin, Streptozotocin, Acute toxicity, Oxidative stress.