Original Research Article
Year: 2016 | Month: October | Volume: 6 | Issue: 10 | Pages: 57-62
Transcriptomic and Proteomic Expression Analysis of Cysteine Rich Angiogenic Inducer 61 in Normal and Diaphyseal Tibial Fractures Patients: A Case Control Study
Manish Yadav1*, Ajai Singh2*, Sabir Ali3*, Abbas Ali Mahdi2**
1Senior Research Assistant, 2Professor, 3Ph.D. Scholar
*Department of Orthopaedic Surgery, **Department of Biochemistry,
King George's Medical University, Lucknow, Uttar Pradesh, India- 226003.
Corresponding Author: Ajai Singh
ABSTRACT
Angiogenesis is the process of the formation of new blood vessels from pre-existing vasculature. Angiogenesis is a prerequisite for fracture repair. Cysteine Rich Angiogenic Inducer 61 (CYR61) is one of the markers responsible for angiogenesis as well as chondrogenesis. In the present case-control study, we aimed to analyse the transcriptomic and proteomic expression of CYR61 in normal (control) and patients with diaphyseal tibial fractures. A total 129 cases with simple, fresh traumatic diaphyseal fractures of both bones of the leg managed conservatively and 129 healthy controls enrolled. Samplings of blood were taken at 4th day of fracture in cases and in controls. The CYR61 mRNA and protein expressions were quantifies by using qRT-PCR and western blotting assay technique. In this study, we found significant difference in expression level of both CYR61 mRNA and protein between cases and controls. Simultaneously, as the difference in expression obtained in early phase of fracture healing, we conclude that the CYR61 might be used as a potential biomarker to predict the fracture healing outcomes early.
Key words: CYR61; Angiogenesis; Angiogenic Marker; Diaphyseal tibial fractures, Bimarker.