Year: 2024 | Month: September | Volume: 14 | Issue: 9 | Pages: 256-259
DOI: https://doi.org/10.52403/ijhsr.20240933
Detection of Metallo-Beta-Lactamase Encoding Genes by Multiplex RT-PCR among Clinical Isolates of Pseudomonas Aeruginosa
Deepa Upadhyay1, Dr. Anshu Sharma2
1Ph.D. Scholar, Department of Microbiology, RNT Medical College, Udaipur, Rajasthan, India.
2Senior Professor, Department of Microbiology, RNT Medical College, Udaipur, Rajasthan, India.
Corresponding Author: Deepa Upadhyay
ABSTRACT
Introduction: Metallo-beta-lactamase producing Pseudomonas aeruginosa is an important emerging nosocomial pathogen, due to its ability to acquired drug resistance.
Aim & objectives: The aim of this study was to detect MBL encoding genes by genotypic method among clinical isolates of Pseudomonas aeruginosa.
Material & Method: A total of 50 non-repetitive isolates of P. aeruginosa recovered from various clinical samples were included in this study. All these isolates were screened for MBL production by RT-PCR irrespective of imipenem sensitivity and the presence of blaVIM, blaIMP & blaNDM was determined.
Results: Out of 50 isolates of P. aeruginosa, MBL were detected in 32 (64%) isolates by PCR. blaVIM gene was detected in 21 isolates, followed by blaNDM in 11 isolates and blaIMP in 5 isolates.
Conclusion: The prevalence of MBLs among Pseudomonas aeruginosa has been increasing. This study confirms a high rate of MBL production & dissemination of blaVIM gene among Pseudomonas aeruginosa.
Key words: Pseudomonas aeruginosa, Metallo-beta-lactamase, blaVIM, blaIMP, blaNDM, PCR.