Original Research Article
Year: 2023 | Month: August | Volume: 13 | Issue: 8 | Pages: 15-22
DOI: https://doi.org/10.52403/ijhsr.20230803
In Vivo Anticancer Activity of Snuhi Kshara Against 1,2-Dimethyl Hydrazine Induced Colon Cancer
Wadhai Sayli Waman1, Nikam Ashwin Vithalrao2, Pawade Uday Venkatrao3, Anjankar Meghsham Pramod4
1MD Agadtantra, Shri Ayurved Mahavidyalaya, Maharashtra University of Health Sciences, Nagpur, Maharashtra, India
2HOD and Professor, Department of Agadtantra, Shri Ayurved Mahavidyalaya, Maharashtra University of Health Sciences, Nagpur, Maharashtra, India
3Associate Professor, Department of Agadtantra, Shri Ayurved Mahavidyalaya, Maharashtra University of Health Sciences, Nagpur, Maharashtra, India
4Assistant Professor, Department of Agadtantra, Shri Ayurved Mahavidyalaya, Maharashtra University of Health Sciences, Nagpur, Maharashtra, India
Corresponding Author: Wadhai Sayli Waman
ABSTRACT
Background- Colon cancer is the third most diagnosed cancer globally with incidence rate 1.15 million new cases. Snuhi (Euphorbia neriifolia Linn.) possesses anti-inflammatory, anti-ulcer, antioxidant, immunomodulatory, cytotoxic and anticarcinogenic properties as per previous studies.
Objective- The present study aimed to explore anticancer activity of Snuhi Kshara (SK), the formulation of upavisha snuhi against colon cancer in Wistar rats.
Materials and methods- 1,2-dimethylhydrazine (DMH) was used to establish colon cancer model in female Wistar Albino rats. The acute toxicity of Snuhi Kshara was tested in accordance with OECD guidelines. Change in body weight, hematological and biochemical parameter, colon length and weight, Aberrant Crypt Foci (ACF) count, histopathological findings with toxicity score were used to screen In Vivo anticancer activity.
Result- The oral LD50 cut off value of Snuhi Kshara was found to be 5000mg/kg body weight. Snuhi Kshara significantly (p<0.05) increase the body weight and colon length in treated group. Snuhi Kshara significantly (p<0.05) decrease the Total Cholesterol Level (TCL), colon weight, Aberrant Crypt Foci (ACF) count and histopathological toxicity score. Snuhi Kshara decreased the colonic injury induced by 1,2-dimethylhydrazine and helped to repair.
Conclusion- Based on the findings, it can be concluded that Snuhi Kshara possesses anticancer activity against DMH induced colon cancer. Further studies are required to determine the exact mechanism of action of Snuhi Kshara.
Key words: Snuhi (Euphorbia neriifolia Linn.), Snuhi Kshara, Anticancer, Aberrant Crypt Foci (ACF)