Original Research Article
Year: 2022 | Month: April | Volume: 12 | Issue: 4 | Pages: 246-256
DOI: https://doi.org/10.52403/ijhsr.20220428
Early Detection of Blood Borne Pathogens and its Antibiotic Susceptibility
Uday Rajesh Titare1, Dr. Niraj Ghanwate1, Pradnya Saurkar1, Ravindra Ashok Sharma2
1P.G. Department of Microbiology, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India.
2Department of Microbiology, R.K. Talreja College, Ulhasnagar- 421003, Maharashtra, India.
Corresponding Author: Uday Rajesh Titare
ABSTRACT
In this study the evaluation of the new blood culture technique to improve rapid detection for the presence of bacteria in blood by using Tetrazolium dye (TTC) was done. The Blood samples were collected and processed by two different methods i.e. conventional blood culturing method and improved method by using TTC dye. In conventional method the sample was inoculated in blood culture bottle and then plated on blood agar, MacConkey, and chocolate agar and was incubated for 24hrs. After incubation the growth observed was identified and reported. Along with this in Improved TTC dye method the blood was inoculated in tryptone soya broth incubated and then TTC dye was added in it and cherry red color development was considered as positive. In the present study, total 50 blood samples were processed of which 32 samples exhibited growth on media and 18 samples show no growth. From total positive growth samples, 9 were gram positive including Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus epidermidis and remaining 23 were gram negative rods including E. coli, Pseudomonas aeruginosa and Klebsiella pneumonia. Overall, the average time required for growth by conventional method is 51 hours while that of TTC dye method is of 30 hours. In contrast to antibiotic susceptibility test, Microbroth dilution method was easy, reliable, affordable and quick result giving method as compare to standard antibiotic susceptibility test method.
Key words: Bloodstream infections, Antibiotic susceptibility, Tetrazolium dye.