Original Research Article
Year: 2022 | Month: March | Volume: 12 | Issue: 3 | Pages: 390-395
DOI: https://doi.org/10.52403/ijhsr.20220351
Detection of Epstein Barr Virus DNA in Gastric Adenocarcinoma in Brazzaville
Fidele Mambouene1, Dimitry Moudiongui2, Anicet Mboumba3, Yvon Mabiala4, Etienne Mokondjimobe5, Donatien Moukassa6, Jean Félix Peko7, Jean Bernard Nkoua Mbon8, Ange Antoine Abena9
1,2PhD Student Pending Defense in Service at the Pathological Anatomy Laboratory of the University Hospital of Brazzaville
3Doctor, Medical Oncologist of the University Hospital of Brazzaville
4Virologist, Assistant Professor at Marien NGOUABI University
5Anatomopathologist, Professor at the Faculty of Medicine
6Head of Laboratory Department for Biomedical Research and Analysis Training Faculty of Health Sciences
7Professor, Coordinator of the Doctoral School of Health and Human Biology, Faculty of Health Sciences
8Professor, Medical Oncologist of the University Hospital of Brazzaville, Head of Medical Oncology
9Anatomopathologist, Professor at the Faculty of Medicine, Head of Service, Pathological Anatomy Laboratory of the University Hospital of Brazzaville
Corresponding Author: Fidele Mambouene
ABSTRACT
Introduction: The Epstein Barr virus (EBV) is associated with a variety of human malignancies including gastric cancer (GC).
Several studies have shown an association between Epstein-Barr virus (EBV) infection and the occurrence of many cancers in humans, including certain gastric carcinomas (GC). Indeed, recent studies have reported that 10% of GCs are associated with EBV. There is no study on the association of EBV with CG in Brazzaville.
The aim of the study was to detect EBV DNA in gastric adenocarcinoma in Brazzaville.
Materials and Methods: Samples of carcinomatous gastric tissues were analyzed by qPCR to detect EBV DNA. The samples were collected retrospectively between January 2008 and December 2018.
Results: Fifty two samples were analyzed. The PCR results showed that the detection of EBV DNA on our sample was 3.8%.
Conclusion: The results obtained from this preliminary study confirm the presence of EBV DNA in gastric adenocarcinoma, which is consistent with data from the literature.
Key words: DNA, EBV, gastric adenocarcinoma, qPCR.