Original Research Article
Year: 2022 | Month: October | Volume: 12 | Issue: 10 | Pages: 19-29
DOI: https://doi.org/10.52403/ijhsr.20221003
In Vitro Anticancer Activity of Kokilaksha (Hygrophila Spinosa T Ander.) in Osteosarcoma Cell Lines
Anisha E.P.1, Pradeep Seema2, PM Manjunatha3
1PG Scholar, Department of PG Studies in Dravyaguna, Sri Sri College of Ayurvedic Science and Research, Bengaluru, India
2Professor and HOD, Department of PG Studies in Dravyaguna, Sri Sri College of Ayurvedic Science and Research, Bengaluru, India
3Professor and HOD, Department of Pharmacology, Acharya & BM Reddy College of Pharmacy, Bengaluru,India
Corresponding Author: Dr. E.P. Anisha
ABSTRACT
Introduction: The drug Kokilaksha (Hygrophila spinosa T Ander.) found growing as a weed in the paddy fields is one of the commonly used drugs to treat Vatarakta (Gouty arthritis) as per classical texts of Ayurveda. A thorough literary search on the disease Vatarakta reveals that in Gambhira awastha (chronic stage) the disease involves deeper dhatus like Sandhi, Asthi, Majja; and Arbuda is mentioned as one of the upadravas (complications) of Gambhira Vatarakta according to Acharya Sushruta. Both malignant as well as benign tumours are considered under the term Arbuda. The research profile of Kokilaksha reveals its potent anti-tumour activity and efficacy in the Erythropoietic system. The current study is intended to evaluate the in vitro anticancer activity of Kashaya and hydroalcoholic extract of the drug Kokilaksha (Hygrophila spinosa T Ander.) in osteosarcoma cell lines.
Materials and Methods: The in vitro study consisting of two osteosarcoma cell lines namely MG-63 and SAOS-2 were subjected to the hydro alcoholic extract and kashaya of Kokilaksha (Hygrophila spinosa T Ander.). The anticancer activity was carried out using three assays namely MTT assay, Nitric oxide scavenging activity and LDH assay.
Results: The in vitro study showed both the hydro alcoholic extract and kashaya of Kokilaksha (Hygrophila spinosa T Ander.) exhibited anticancer activity in a time and concentration dependent manner with respect to all the three assays in both MG-63 and SAOS-2 osteosarcoma cell lines.
Key words: Kokilaksha (Hygrophila spinosa T Ander.), osteosarcoma cell lines, MTT assay, Nitric oxide scavenging activity, LDH assay, hydro alcoholic extract, Kashaya