Original Research Article
Year: 2021 | Month: September | Volume: 11 | Issue: 9 | Pages: 259-264
DOI: https://doi.org/10.52403/ijhsr.20210939
Establishment of Reference Values of Gamma-Glutamyl-Transferase and Plasma Aminotransferases in Young Congolese
Linel Darrel Ngokana1, Fylla Onanga Koumou1,2, Landry Aymar Loukanou Mbouaka1, Childerick Lékana1, Reine Freudlendrich Eboka Loumingou Sakou4, Edouard Ngou-Milama7, Aliocha Nkodila Natuhoyila5,6, Benjamin Longo Mbenza5,6, Martin Diatewa1,3, Etienne Mokondjimobe1,4,6
1Laboratory of Biochemistry-Pharmacology, Faculty of Health Sciences, Brazzaville, Congo
2Biochemistry Laboratory, University Hospital Center, Brazzaville, Congo
3Direction of Scientific Research of the Marien NGOUABI University, Brazzaville, Congo
4Clinical Biochemistry Laboratory, National Public Health Laboratory, Brazzaville, Congo
5Department of Medicine, Faculty of Medicine, UNIKIN, Kinshasa, DRC
6Lomo University of Research, Kinshasa, DRC
7Faculty of Health Sciences, Omar Bongho Ondimba University, Libreville, Gabon
Corresponding Author: Aliocha Natuhoyila Nkodila
ABSTRACT
Objectives: Our goal was to contribute to the production of reference values of plasma or serum biochemical markers by determining the reference values of gamma-glutamyltransferase (GGT), aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) in young Congolese presumed to be healthy.
Methods: 250 young Congolese presumed to be healthy (125 boys and 125 girls) aged 15 to 25 participated in the study. They were selected according to anamnestic and clinico-biological criteria. Samples were taken on a tube containing EDTA and the resulting plasma was stored at -20 ° C. The KENZA MAX spectrophotometer was used to analyze GGT, ASAT and ALAT. The median and the 2.5-97.5 percentiles were used to set the reference limits for each enzyme. The benchmarks determined were compared with those reported by other Africans, Europeans, Indians and Americans.
Results: The established reference values were: GGT 12.15-61.85 IU/L for boys and 7-51.95 IU / l for girls (p˂0.0001); ASAT 21.60-94.85 IU/L for boys and 17-84.85 IU/L for girls (p = 0.0003); ALAT 8.30-74.40 IU/L for boys and 8-53.85 IU/L for girls (p˂0.0001). In addition, the comparison between our values and those of other populations showed significant differences.
Conclusion: Our results underline the importance of establishing reference values for plasma enzymes specific to the Congolese population. The use of the values established in the ’other populations could induce errors of judgment by excess or by default.
Key words: Gamma-glutamyltransferase, Aspartate aminotransferase, Alanine aminotransferase, Reference values, Congo.