Original Research Article
Year: 2021 | Month: November | Volume: 11 | Issue: 11 | Pages: 180-190
DOI: https://doi.org/10.52403/ijhsr.20211124
Molecular Epidemiology of Epstein-Barr Virus in Women Breast Cancer in Congo Brazzaville
Dimitry Moudiongui Mboungou Malanda1,2,5, Anicet Luc Magloire Boumba1,3, Fabien Gaël Mouamba1,2, Landry Martial Miguel1,2,5, Donatien Moukassa1,4, Jean Félix Peko1,2, Ange Antoine Abena1,2,3
1Faculty of Health Sciences, Marien Ngouabi University of Brazzaville. 5Oncology Department, Hospital.
2Anatomy and Pathological Cytology Department, Brazzaville Hospital and University Center (CHUB).
3Laboratory of Medical and Morphological Analysis, General Hospital of Loandjili in Pointe-Noire (HGL).
4Pathological Anatomy and Cytology Department, Edith Lucie Bongo Ondimba General Hospital in Oyo (HGELBO).
5Dénis University Sassou Nguesso
Corresponding Author: Dimitry Moudiongui Mboungou Malanda
ABSTRACT
Introduction: the Epstein Barr virus is one of the very first oncogenic viruses to be identified as responsible for human malignancies. Its role as an etiological agent of breast cancer remains controversial, however, despite the growing molecular evidence. The aim of this study was detected the presence of EBV DNA in patients with breast cancer in the Republic of Congo.
Methods: The study was conducted on 90 samples of formalin fixed and paraffin-embedded tissue blocks (FFPE) from breast cancer tissue. The immunohistochemistry technique was used to test for the expression of the LMP1 antibody and DNA was extracted from all blocks of formalin-fixed and paraffin-embedded breast cancer tissue (FFPE) to detect presence of EBV 1 DNA by real-time polymerase chain reaction (PCR).
Results: EBV was detected in 12.33% (12/90) of formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks. All formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks with positive EBV DNA were high tumor grades (II and III). Overall EBV infection with clinicopathological features of breast cancer cases showed no significant difference (P>0.05). However, a statistically significant difference was observed between EBV infection and histological types (P=0.04).
Conclusion: Our results provide evidence for the presence of EBV DNA in female breast cancer in Congo Brazzaville. However, this evidence is substantial but inconclusive for the involvement of viruses in the development of breast cancer. Therefore, future investigations will be needed to elucidate the exact role of EBV in breast cancer in women in the Republic of Congo.
Key words: EBV, breast cancer, women, Congo Brazzaville.