Original Research Article
Year: 2020 | Month: July | Volume: 10 | Issue: 7 | Pages: 239-246
Evaluation of Some Antioxidants in tuberculosis Patients
Ihim Augustine Chinedu1, Meludu Samuel Chukwuemeka1,2, Onyenekwe Charles Chinedum1, Onah Christian Ejike1
1Chemical Pathology Unit, Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State , Nigeria.
2Department of Human Biochemistry, Faculty of Basic Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra state, Nigeria.
3Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus
Corresponding Author: Ihim Augustine Chinedu
ABSTRACT
Objective: Serum levels of vitamins C, E, selenium , malondialdehyde(MDA), activities of superoxide dismutase(SOD), catalase, glutathione peroxidase(GPx) and glutathione reductase (GRx) in individuals with Mycobacterium tuberculosis (MTB) infection was investigated.
Methods: 251 individuals consisting 120 treatment naïve individuals with active TB [26 (TB+HIV+) and 12 malaria parasite (MP) and TB (TB+MP+) co-infection, 82 HIV negative (TB+HIV−)], 26 Latent TB (LTB) and 105 apparently healthy control (AHC). TB infection was determined by Ziehl-Neelsen sputum smeared microscopy and gene Xpert. MP was confirmed by Giemsa staining technique, HIV by immuno-chromatographic method. Analytes were evaluated spectrophotometrically, but selenium was by atomic absorption spectrometer. SPSS version 21 ANOVA was used for statistical analyses.
Results: The mean levels of vitamins E, C and selenium were significantly lower in individuals with TB infections compared with AHC (p<0.05). Significantly higher differences were observed in MDA levels in TB infections than in AHC (p<0.05). Significantly lower differences existed in the mean levels of vitamin C and selenium in LTBI than in control, MDA was significantly higher in the same group (p<0.05). The mean levels of vitamin E in TB+HIV+ (1.57±1.30) and TB+MP+ co-infections (2.23±1.70) were significantly lower compared with those with TB+(7.01±3.40)(p<0.05).The mean SOD, catalase and GPx activities in active tuberculosis, TB+HIV+ and TBMP co-infections were significantly lower than in the control (p<0.05). The mean GRx activity in AHC, active TB, TB+HIV+ and TB+MP+ co-infections were significantly lower than in LTBI(p<0.05).
Conclusions: Glutathione reductase may have prognostic potential in the differential screening for latent tuberculosis.
Key words: Antioxidants; Tuberculosis; Malondialdehyde.