Original Research Article
Year: 2020 | Month: June | Volume: 10 | Issue: 6 | Pages: 30-35
Cytotoxicity Evaluation of Swasanandam Gutika by MTT Assay in Mouse Fibroblast Cells
Sugeena P1, Tharalakshmi S2
1Assistant Professor, Department of Rasasastra and Bhaishajyakalpana, KMCT Ayurveda Medical College, Manasserry, Kozhikode, Kerala
2Associate Professor, Department of Rasasastra and Bhaishajyakalpana, Government Ayurveda College, Thiruvananthapuram, Kerala
Corresponding Author: Sugeena P
ABSTRACT
Introduction: Swasanandam gutika (SG) is one among the popular rasa formulation mentioned in Arogyakalpadruma, a textbook on balachikitsa. SG is a pill formulation containing Hingula, Vatsanabha and karpoora levigated in Triphalakwatha. Both hingula and vatsanabha are toxic drugs included in the schedule E1 of Drug and cosmetic act 1940. Also camphor is reported to be having toxicity effects. SG is generally administered to children in respiratory illness and hence there is a need of toxicity profiling to ensure safety. Heavy metals like mercury are reported to be having cytotoxic effect. So to evaluate the cytotoxicity of this formulation is an important matter of concern. Aims and objectives: To evaluate the in-vitro cytotoxicity of Swasanandam gutika on mouse fibroblast cells.
Materials and methods: SG was prepared and cytotoxicity was assessed by MTT assay in L929 cell line.
Results: The cell viability at different concentrations are measured. The viability of cells was found to be reduced on increasing the concentration of the drug.
Conclusion: It can be concluded that the drug is safe and non-cytotoxic at concentration less than 125μg/ml.
Key words: Swasanandam gutika, In-vitro cytotoxicity, MTT assay, L929 cells