Original Research Article
Year: 2020 | Month: December | Volume: 10 | Issue: 12 | Pages: 193-199
Assessment of Adiponectin Gene Variants and Protein Level in Osteoarthritis among Egyptian Population
Fathy M. Elfasakhany1,2, Ayman A. Khalil3, Magdi A. Eldamarawi4,5
1Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia
2Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt
3Department of Orthopedics and Traumatology, Faculty of Medicine, Tanta University, Tanta, Egypt
4Department of Medical physiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
5Department of Medical physiology, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia.
Corresponding Author: Fathy M. Elfasakhany
ABSTRACT
Background: Osteoarthritis (OA) is a common joint disease influencing the majority of individuals over the age of 65. The most affected joints in OA are knees, hands, hips, and spine leading to impaired mobility in the elderly.
Objectives: To analyze and correlate the level of plasma adiponectin and the adiponectin G/T (rs1501299) single nucleotide polymorphism in a group of Egyptians with knee osteoarthritis (OA) and a control group.
Materials and methods: 130 unrelated subjects with knee OA and 120 healthy subjects were enrolled in this study. Peripheral blood was used for extraction of DNA which was used to genotype adiponectin gene G/T (rs1501299) polymorphism utilizing polymerase chain reaction followed by digestion with restriction endonuclease. Plasma adiponectin level was estimated using enzyme linked immunosorbent assay (ELISA).
Results: Plasma adiponectin concentration was higher in the OA subjects than the controls (P = 0.01). For the adiponectin G/T (rs1501299) polymorphism, there was no significant difference in the genotype distribution and allele frequency of the adiponectin between the control subjects and the OA subjects (P > 0.05).
Conclusion: The results suggested adiponectin G/T (rs1501299) polymorphism might not be related to the susceptibility of OA among the Egyptian subjects.
Key words: gene polymorphism; osteoarthritis; adiponectin; Polymerase chain reaction; restriction enzyme; ELISA.