International Journal of Health Sciences and Research

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Original Research Article

Year: 2019 | Month: March | Volume: 9 | Issue: 3 | Pages: 105-118

Evaluation of Antimicrobial Activities of Commercial Product (RH5+) and its Competitor Products against Clinically Isolated Bacterial Strains and Bacterial Population Present in Poultry Bed

Jhimli Banerjee1, Sananda Dey1, Hasnin Nigar Rizowana1, Sovan Samanta1, Rimee Dutta1,2, Pratip Kundu3, Labanyamoy Kole2*, Biplab Giri1*, Sandeep Kumar Dash1*

1Department of Physiology, University of Gour Banga, Malda 732103, India
2R.R Animal Health Care Ltd, Adarsh Nagar Colony, Nagole, Hyderabad, Telangana 500068, India
2Principal, Malda Medical College and Hospital, Malda 732101, India

*Corresponding Authors: Labanyamoy Kole, Biplab Giri, and Sandeep Kumar Dash


The gradual increase of antibiotic resistance in microbes has become the greatest health threat for human beings and animals. Disinfectants which are generally used in the farming industry effected on animal health and reduced productivity. Therefore, the aim of the present study was to evaluate antimicrobial activity of newer marketed disinfectant product RH5+ and its three competitor products (D1, D2 and D3) against clinically isolated multidrug resistant bacteria Escherichia coli (EC-1), Pseudomonas aeruginosa (PA-1), Klebsiella oxytoca (KO-1), Proteus vulgaris (PV-1), and Staphylococcus aureus (SA-1) and also investigate their efficacy against the bacteria found in Poultry bed. RH5+, D1, and D3 showed excellent antimicrobial efficacy against clinical isolates as well as against the bacterial populations found in the soil of poultry bed. The MIC, MBC values were noted at very low concentration (1µl/mL) of the tested compounds and inhibition zones were also significant as observed from agar diffusion assay. The RH5+, D1 and D3 also reduced bacterial colony formation from poultry bed soil. The D2 did not show any antimicrobial property against clinical isolates up to 100µl/mL dose. The quaternary ammonium, glutaraldehyde and other active ingredients of the commercial compounds present in those formulations were noted as potent bacteriostatic against multidrug resistant bacteria and against the bacterial populations found in the poultry bed soil. Thus, it was concluded that the commercial products i.e. RH5+ and competitor products could be used as an effective disinfectant in poultry farm to eliminate the pathogenic infections of the birds.

Key words: Commercial antimicrobial, RH5+, minimum inhibitory concentration, minimum bactericidal concentration, poultry bed

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