Original Research Article
Year: 2019 | Month: February | Volume: 9 | Issue: 2 | Pages: 115-121
Identification and Characterization of Lectins from Leguminosae Plants
Soumya Purkait1, Shyamal Koley2
1MYAS GNDU, Department of Sports Sciences and Medicine, Guru Nanak Dev University, Amritsar - 143005, Punjab, India
2Department of Physiotherapy, Guru Nanak Dev University, Amritsar - 143005, Punjab, India
Corresponding Author: Soumya Purkait
ABSTRACT
Introduction: Since lectins are widely found in Leguminosae family due to their high protein content, in the present study, an attempt has been made to identify such legume lectins which have the agglutination properties with the red blood cells from normal individuals.
Methods: Normal blood samples were collected, using the finger-prick technique. All blood samples were washed thrice in physiological saline and re-suspended at a concentration of 2% in normal saline. For ABO typing, standard serological procedure were followed.
Results: In the results, various lectins showed the hemagglutination reaction pattern with human ABO blood groups. The lectin reacted with various blood groups with the strength of 1:1, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, and 1:128.
Conclusion: The research findings were considered as the first step towards the field of sports nutrition. The lectin seeds are collected from the daily routine of the sports person, without knowing the effect of seeds on the body. Properly denaturized seeds should be consumed.
Key words: Lectins. Hemagglutination. ABO blood types.