Original Research Article
Year: 2018 | Month: September | Volume: 8 | Issue: 9 | Pages: 52-56
Quantitative Analysis of Apoptotic Cells in Normal Mucosa, Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma Using Methyl Green-Pyronin Stain
Ani Simila. CS1, Isaac Joseph. T2, Prasanth. T2, Girish. KL2
Department of oral pathology, Sree Mookambika Institute of Dental Sciences, Kulasekharam.
Corresponding Author: Girish. KL
Background: Apoptosis is a form of physiologic cell death which involves coordinated events and responsible for mediating cell death in a variety of physiologic and pathologic process. Apoptotic cells can be identified by routine haematoxylin and eosin staining, but can be identified with more precision by methyl green-pyronin staining. The added advantage of this stain is that it is simple and cost effective method as compared to other advanced diagnostic methods available for identifying apoptotic cells.
Aim: To identify the expression of apoptotic cells in normal epithelium, oral epithelial dysplasia and in oral squamous cell carcinoma using methyl green-pyronin stain.
Materials and methods: The study included a total of thirty samples. Ten cases each of oral epithelial dysplasia and oral squamous cell carcinoma made up the study group whereas, ten cases of normal mucosa constituted the control group. Apoptotic cells were identified and apoptotic index was calculated using methyl green-pyronin stain. Results were statistically analysed and compared between the groups.
Results: The mean apoptotic index was found to be increased from normal mucosa to oral squamous cell carcinoma. Oral squamous cell carcinoma showed higher mean apoptotic index (3.89±0.63) followed by oral epithelial dysplasia (2.06±0.13) and normal mucosa (1.50±0.28).
Conclusion: Methyl green-pyronin staining method can be used as a routine stain in laboratory in identifying the apoptotic cells.
Key words: Apoptosis, Oral epithelial dysplasia, Oral squamous cell carcinoma, Methyl green-pyronin stain