Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 56-61
Assessment of Rapidec Carba NP Test, as Screening Test to Detect Carbapenemase, Specially Metallo Β Lactamase Producing Gram Negative Bacteria at Tertiary Care Centre
Dr.Kalpana.Tikaram.Suryavanshi1, Dr.Kunal.Kanti.Lahiri2
1Assistant Professor, 2Professor, & H.O.D. Department of Microbiology,
Bharati Vidyapeeth Deemed To Be University & Medical College, Pune.
Corresponding Author: Dr.Kalpana.Tikaram.Suryavanshi
ABSTRACT
Rapidec Carba NP test is recommended by Clinical laboratory Standard institute (CLSI) for detection of carbapenemase.
Metallo β lactamase (MBL), especially NDM1 are increasingly detected in Indian health care settings. Routine phenotypic test to detect MBL requires about 48 hours & other tests including molecular test are laborious & not rapid. So present study was undertaken to assess the utility of Rapidec Carba NP test in detection of Carbapenemase & MBL producing Gram Negative bacilli in paediatric patients in comparison to phenotypic test.
Aim & Objective: To assess the utility of Rapidec Carba NP test to detect carbapenemase, specially, MBL producing Gram Negative Bacilli & carbapenemase to know the occurrence of MBL in paediatric patients.
Settings and Design: Prospective, laboratory based cross sectional study was conducted at Microbiology Laboratory of Tertiary Care centre.
Methods and Material: Fifty Carbapenem Resistance Gram negative isolates from paediatric patients identified by Vitek 2 Compact automation system were further tested for MBL production, using phenotypic test. All isolates including MBL positive were subjected to Rapidec Carba NP Test.
Statistical analysis used: Statistical package of social science (SPSS) version 22.0 software.
Results: 82% carbapenem positive isolates were detected by Rapidec Carba Np Test out of fifty isolates. Twenty isolates were identified as MBL positive, eighteen of which were correctly identified by Rapidec Carba Np test giving sensitivity of 90%.
Conclusions: With 90% sensitivity &Turnaround time of 30 minutes to 2 hours, Rapidec Carba NP test proves effective screening test in adjunct with other phenotypic test. Forty percent carbapenem resistant isolates were MBL positive in Paediatric patients.
Key words: Rapidec Carba NP test, CLSI, Metallo-beta-lactamase, Paediatric.