Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 129-135
Extraction Efficiency of Agnuside from Vitex Negundo Leaves Using Different Techniques and its Quantitative Determination by HPLC
S.P. Patil, K.S. Laddha
Medicinal and Natural Product Research Laboratory, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, India-400 019.
Corresponding Author: Sapna P. Patil
Objective: To investigate an efficient extraction technology and HPLC method for quantification of agnuside from Vitex negundo methanolic extract.
Methodology: Different extraction methods have been compared that includes reflux, ultrasonication, maceration and microwave extraction techniques which were evaluated for their efficiencies. For reliable quantification of agnuside, HPLC was done using solvent system, acetonitrile and water containing 0.5% OPA at wavelength 254nm and flow rate of 1ml/minute. Based on HPLC analysis the best extracting method was selected. The developed method was applied for evaluation of extracts as well as formulations like tablets and capsules.
Results: Among the four conventional methods microwave assisted technique, showed highest extraction efficiency for agnuside hence it was further evaluated. The calibration plot of standard agnuside showed a linear relationship in the concentration range of 20-100 µg/mL with a correlation coefficient, r2 of 0.999.
Conclusion: The maximum yield for agnuside was obtained by microwave assisted extraction technique. The HPLC method was found to be linear having high precision, accuracy and good recovery of the compound.
Key words: Agnuside, HPLC, Microwave, Reflux, Validation, Vitex.