Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 110-114
Expression of Vascular Endothelial Growth Factor-A 165 (VEGF-A) in Term Normal Placenta: Immunohistochemical Study
Vanitha1, Dr. Daksha Dixit2, Dr. Adarsh Sanikop3, Dr. R. D.Virupaxi4
1Ph. D. Scholar, 2Professor, 4Professor& Head, Department of Anatomy,
3Assistant Professor, Department of Pathology,
Jawaharlal Nehru Medical College, KLE Academy of Higher Education & Research (KAHER), Belagavi, Karnataka, India.
Corresponding Author: Vanitha
Introduction: Vascular Endothelial Growth Factor (VEGF) is a potent angiogenic growth factor and acts as a key regulator of vascular development, implicated in the development of both pathological and physiological angiogenesis. In the placenta, it has been identified recently and considered as one of the important angiogenic growth factors in the development of the embryo. We have taken up this study to see the expression of VEGF-A 165 in normal placenta by immunohistochemistry.
Materials and methodology: One hundred central sections of placentae slides were deparaffinized followed by peroxidase block and protein block. After this, the slides were incubated in VEGF mouse monoclonal antibody for 1 hr followed by incubation in Horseradish peroxidase (HRP) for 30 mins and Diaminobenzidine (DAB). Stained sections were observed for VEGF expression in the villi of placental tissues.
Results: Expression of VEGF was observed in syncytiotrophoblast, endothelium of blood vessels and hofbauer cells of villi of the placenta.
Conclusion: In this study, we studied the expression of VEGF in normal term placenta of 37-41 weeks of gestation. The VEGF expression on syncytiotrophoblast and hofbauercells can act on paracrine mode on vessels to modulate angiogenesis, to maintain endothelium and permeability and through autocrine mode to influence trophoblast function during pregnancy.
Key words: Placenta, terminal villi, VEGF.