Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 89-97
Comparative Evaluation of Antibacterial Efficacy of Cannabis Sativa, Allium Sativum, Allium Cepa, Thuja Orientalis and Psidium Guajava against Drug Resistance Pathogens
Nashra A1, Sujatha R2, Deepak Sameer3, Bagoliwal A4,Vikas Mishra5, Anil Kumar6, Majid A7
1Ph.D. Scholar, 2Professor and Head, 3Post Graduate, Department of Microbiology,
4Professor, Department of Community medicine, 6Assistant Professor, Central Research Laboratory,
Rama Medical College Hospital and Research Centre, Mandhana, Kanpur (UP) India
5Assistant Professor, Department of Microbiology, GSVM, Medical College Kanpur (UP) India
7Incharge NICU, Madhuraj Hospital, Kanpur.
Corresponding Author: Sujatha R
ABSTRACT
Background: The increase in isolates of S. aureus with resistance to methicillin and decreased susceptibility to vancomycin has created an urgent and consideration need for the development of new anti-staphylococcal agents that kill the resistant mutants. So, the identification of an alternative and safer drug for the control of MRSA is necessary to combat this worldwide problem. Nature is the only source to provide a good variety of chemical compounds that can be used for new drug discovery especially from mineral, plant and animal products.
Methods: Clinical strains were isolated from hospitalized patients in Rama Medical College Hospital and Research Centre Mandhana, Kanpur. The ethanolic extracts of plants Cannabis sativa, Allium sativum, Allium cepa, Thuja orientalis and Psidium guajava was taken, of which 1g was prepared with 5% and 10% concentration respectively. The antimicrobial properties of the extracts were determined through disk diffusion method.
Results: The sensitivity of bacteria was detected based on the disk diffusion method, the zone of inhibition was obtained for VRSA (20mm), VISA (19mm), MRcons (32mm), MRSA (20mm), MSSA (22mm) and ATCCS.aureus25923 (23mm) at different concentration of the plant extract. The highest inhibition zone of plant extract was observed in Cannabis sativa followed by Allium sativum then Allium cepa, Psidium guajava and lastly by Thuja orientalis. It was also observed that the zone of diameter increased with the increase of concentration.
Conclusion: The plants included in this study showed mild to moderate anti-microbial activity. This further concluded that these plants can be used as a potent agent for the treatment of MDR infections.
Key words: Antibiotic resistance, plant extracts, MDR infections.