Original Research Article
Year: 2018 | Month: July | Volume: 8 | Issue: 7 | Pages: 50-54
Laboratory Evaluation of a New Rapid Slide Culture (RSC) Technique for Diagnosis of Extra-Pulmonary Tuberculosis
Singh DN1, Sujatha R2, Meghwani MK3, Bagoliwal A4, Nigam S. K5, Anil Kumar6
1Ph.D. Scholar, Department of Microbiology, 2Professor and Head, Department of Microbiology,
3Professor, Department of T.B. and Chest, 4Professor, Department of Community Medicine,
5Professor, Department of Pathology, 6Assistant Professor, Central Research Laboratory,
Rama Medical College Hospital and Research Centre, Mandhana, Kanpur, (UP) India.
Corresponding Author: Sujatha R
ABSTRACT
Introduction: Tuberculosis is an infectious disease caused mostly by M. tuberculosis. The bacterium mostly affects lungs but it can affect other parts of the body also. Diagnosis of pulmonary TB is easier than diagnosis of extra-pulmonary TB (EPTB) because of large number of large number of mycobacterium present in sputum sample. Diagnosis of EPTB is difficult depending on liquid culture i.e. MGIT and molecular methods. These methods are time consuming and costly so we have tried a new slide culture method which is cost effective and less time taking.
Materials and Methods: Total 100 patients of suspected EPTB were included in this study after written consent. Menstrual blood, lymph node biopsy and urine samples were subjected for the study of cytology, smear microscopy, MGIT and four sets of RSC.
Result: Out of 100, 24 lymph node biopsy and 13 genital samples were found positive for granuloma cells. Direct smear microscopy has showed 5 AFB positive. Total 11 samples were found culture positive by MGIT culture method. RSC has showed 4 positive cases. Sensitivity and specificity of RSC were recorded 36.4% and 100% respectively. Positive Predictive value and negative predictive value were 100% and 92.5% respectively.
Conclusion: Earlier RSC was used for detection of Mycobacterium growth in only sputum samples. After some improvement we used it for diagnosis of EPTB. In present study the sensitivity of RSC was to 36.4%, which is not acceptable for diagnosis of EPTB but positive predictive value (100%) leaving a hope to improve the RSC technique for diagnosis of EPTB.
Key words: Rapid Slide Culture, RSC, extra-pulmonary TB, EPTB, Tuberculosis.