Original Research Article
Year: 2018 | Month: July | Volume: 8 | Issue: 7 | Pages: 34-40
Screening of the Sap1 Gene of Candida Albicans in Oropharyngeal Cancer Patients in Tertiary Care Unit of Kanpur
Ramesh Yadav1, R Sujatha2, Ajay Bhagoliwal3, Anil Kumar4
1Ph.D. Scholar, 2Professor and Head,
Department of Microbiology, Rama Medical College and Research Centre, Mandhana Kanpur (UP) India.
3Professor, Department of Community Medicine, 4Assistant Professor, Central Research Laboratory,
Rama Medical College, Hospital and Research Centre, Mandhana, Kanpur (UP) India.
Corresponding Author: Anil Kumar
ABSTRACT
Background: In the oral cancer patients because of the immune-suppression there is high risk of Candida albicans infection. The aim of this study was to identify presence of C. albicans in the oral mucosa of 150 patients in Kanpur population.
Methods: This is a prospective study from the oral and maxillofacial OPD of Rama hospital and Research Centre, Kanpur. Swabs were collected and cultured into Sabouraud dextrose agar medium. Presence of Candida species was confirmed microscopically as well as biochemically according to standard procedure. The presence of SAP1 gene was confirmed by isolating RNA and preparing cDNA followed by cDNA amplification by agarose gel electrophoresis.
Results: C. albicans was found the most common species in oropharyngeal cancer patients (42%). The presence of SAP1 was confirmed in the 24 isolates (8%).
Conclusions: Detection of SAP1 gene can help in the treatment and prognosis of C. albicans in the immune-compromised patients especially in oropharyngeal cancer patients.
Key words: Oropharyngeal cancer, Virulence, Candida albicans.