International Journal of Health Sciences and Research

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Original Research Article

Year: 2018 | Month: July | Volume: 8 | Issue: 7 | Pages: 28-33

Screening of Critical Care Setting for Bacterial Colonization

Arth Nath Dube1, Dr. S.A. Samant2, Dr. Mohammad Nasim1

1Resident, Medical Microbiology, MGM Medical College, Kamothe, Navi Mumbai
2Associate Professor, Dept. of Microbiology, MGM Medical College, Kamothe, Navi Mumbai

Corresponding Author: Arth Nath Dube


Hospital acquired infections are a serious problem in patient care and they adversely affect the mortality and morbidity. Mainly the affected areas are critical care units and acute wards where the patients are critical and immuno compromised. In the ICU, the accumulation of a number of immuno compromised patients, their nursing and invasive procedures provide a favourable environment to the growth and transmission of nosocomial infections. The environment of critical care unit and health care workers play an important role in transmission of nosocomial infections Air conditioning vents, ventilator circuits ,door handles, medicine trolley, suction apparatus and mobile phones etc. get colonized with bacteria and disseminated in the hospital which lead to nosocomial infections. The present study is done to isolate and identify pathogenic bacteria from the surfaces of various objects and instruments in the ICU and to carry out resistotyping of the pathogenic bacteria isolated from the Intensive care unit. Swabs were collected from various places of ICUs. Door knobs and mobile phones of Health Care Worker’s showed maximum colonization of GPCs and GNBs. E.coli (12.08%) was the most commonly isolated organism from the ICUs followed by Acinetobacter (8.75%), Pseudomonas (7.5%) and Klebsiella (6.25%). Amongst GPCs Staph. aureus (15%) and CONS (14.16%) were the most predominant colonizers in the ICUs. Aerial bacterial flora of ICUs was studied by exposed plate method which showed by exposed plate method which showed predominant growth of Bacillus spp. and CONS.

Key words: ICUs, HAI, Air Sampling, Resistotyping.

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