Original Research Article
Year: 2018 | Month: July | Volume: 8 | Issue: 7 | Pages: 68-75
Macrophage Chemotactic Protein-1 and Interleukin-12 Levels in Gingival Crevicular Fluid in Patients with Periodontal Disease: A Cross Sectional Study
Njood Alshareef1, Hala A. Abuel-Ela2, Ibtesam K. Afifi3
1Dental intern, Faculty of Dentistry, Umm AlQura University, Saudi Arabia.
2Professor of Periodontology and Implant Dentistry, Faculty of Dentistry, Ain Shams University, Egypt affiliated to Umm-AlQura University, KSA.
3Professor of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt affiliated to Umm-AlQura University, KSA.
Corresponding Author: Njood Alshareef
Background: Periodontal diseases including gingivitis and periodontitis are among the most frequent oral diseases affecting all age groups, which can critically impact the general health. Since periodontal disease is both preventable and curable, early intervention will minimize the subsequent destruction of periodontal tissues.
Objective: Is to assess the gingival crevicular fluid level of Macrophage Chemotactic Protein-1/CCL2 and Interleukin-12 in plaque induced gingivitis patients compared to chronic periodontitis patients.
Methods: This was a cross-sectional study with a sample of 32 healthy female patients obtained from the Dental Teaching Hospital, College of Dentistry, Umm Al-Qura University. GCF samples were collected using PerioPaper Strips.
Results: Chronic periodontitis patients showed statistically significant higher mean PD (5.50mm) than patients with plaque induced gingivitis (3.06mm). Patients with chronic periodontitis revealed greater levels of MCP-1 (0.094pg/ml) in GCF compared to patients with plaque induced gingivitis (0.079pg/ml). Moreover, chronic periodontitis patients showed higher levels of IL-12 (0.11pg/ml) than plaque induced gingivitis patients (0.101pg/ml).
Conclusion: In conclusion, within the limits of the present study, IL-12 and MCP-1 may be regarded as a reliable biochemical marker for periodontal tissue destruction in CP. Further longitudinal studies with larger sample size are recommended to further elucidate the role of these biomarkers in alveolar bone resorption in periodontal disease.
Key words: Chemokines and Gingival crevicular fluid, Chronic periodontitis, Cytokines, Plaque induced gingivitis, Interleukin-12, Macrophage Chemotactic Protein-1/CCL2.