International Journal of Health Sciences and Research

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Original Research Article

Year: 2018 | Month: May | Volume: 8 | Issue: 5 | Pages: 88-91

Bacteriological Analysis of Bile Culture from a Tertiary Care Hospital

Dr. Kanishtha Sharma1, Dr. Beena Jad2, Dr. Bella Mahajan3, Dr. Suharshi Gupta1

1Demonstrator, 2Lecturer, 3Professor and HOD,
Department Of Microbiology, Government Medical College, Jammu, J&K, India.

Corresponding Author: Dr. Kanishtha Sharma


Background: Human bile though sterile normally, can become in¬fected in Biliary tract obstruction due to entry of micro¬organisms through various routes like Papilla of Vater or hematogenous, leading to Bactobilia.
Aim and Objectives: To determine the Bacteriological Profile and Antibiotic Susceptibility Pattern of Bile cultures from a Tertiary Care Hospital.
Setting and Design: A retrospective study was conducted in the Dept. of Microbiology, GMC, Jammu for a period of 1 year i.e. Sep 2016-2017.
Methods: The bile samples were collected in Blood Culture Bottles and incubated for 24 hrs. After that the samples were inoculated on Blood agar and MacConkey agar and incubated overnight at 370C. The isolates were identified by standard microbiological methods. Antibiotic Susceptibility Testing was done by Kirby-Baur Disc Diffusion Method as per CLSI guidelines.
Results: 27 (26.47%) patients showed bile culture positive. The most commonly isolated gram negative organisms were Escherichia coli, Pseudomonas spp and Klebsiella spp. Of the gram positive isolates, Staphylococcus aureus and CONS were obtained. Maximum number of patients were females and in age group 35-40 years. Studying the sensitivity profile, the gram negative isolates were most sensitive to Meropenem, Amikacin and Tobramycin while the gram positive isolates were more sensitive to Linezolid and Vancomycin.
Conclusion: This study concludes that a bile sample for microbiological analysis becomes a valuable diagnostic tool as it leads to more accurate selection of antibiotics for the proper management.

Key words: Bactobilia, CLSI, Microbiological analysis.

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