Original Research Article
Year: 2018 | Month: March | Volume: 8 | Issue: 3 | Pages: 60-69
Acinetobacter Baumannii Complex and Its Beta-Lactamase Production: Are We Moving Towards Pre Antibiotic Era?
Dr. Sanchari Das1, Dr. Silpi Basak2
1Post Graduate Student, 2Professor,
Department of Microbiology, Jawaharlal Nehru Medical College, Wardha (MS), Pin-442004
Corresponding Author: Dr. Silpi Basak
Introduction: Acinetobacter species has been increasingly reported as one of the common cause of nosocomial infections and poses a serious threat to the health care system due to development of its multidrug resistance in debilitated and hospitalized patients. Carbapenem resistance in Acinetobacter sp. is an emerging problem and is a cause of concern as many nosocomial infections with Acinetobacter sp. are resistant to most other antibiotics. The present study was conducted over a period of 6 months in a tertiary care rural hospital to study Acinetobacter baumannii complex strains isolated from clinical samples and their antibiogram.
Materials and Methods: 60 strains of Acinetobacter baumannii complex isolated from various clinical samples like sputum, endotracheal tube secretions, pus, blood etc. were studied. The antimicrobial susceptibility of these isolates was performed by Kirby-Bauer disk diffusion method and Extended Spectrum Beta-lactamase (ESBL), AmpC, Metallo beta lactamase (MBL) and Klebsiella pneumoniae carbapenemase (KPC) production was detected phenotypically.
Results: The maximum sensitivity was seen for Colistin 60 (100%) and least for Ceftazidime 13 (21.6%). MBL production was detected in13.3% Acinetobacter strains. 10% strains produced all 4 types of β-lactamases i.e., ESBL plus AmpC plus MBL plus KPC in combinations.
Conclusion: Proper infection control measures and judicial use of antibiotics is necessary to combat the problem of development of drug resistance. Phenotypic detection of newer β-lactamases should be done routinely in Clinical Microbiology laboratory to prevent development and spread of these β-lactam producing strains in Health care setup.
Key words: Acinetobacter baumannii complex, Antibiotic resistance, Newer β-lactamases,