Original Research Article
Year: 2018 | Month: February | Volume: 8 | Issue: 2 | Pages: 212-220
FTIR-ATR Studies on Phenylhydrazine Induced Hyperbilirubinemia in Wistar Rat
Saira Khatheeja1, Prabhakaran, A.R2, Safiullah A3
1Asst. Professor in Physics Department of Physics, JBAS College for Women, Chennai 600 018,
2Assoc. Professor in Physics Department of Physics, Pachaiyappa’s College, Chennai-600 030,
3Lab Director S. S. Diagnostic Centre, Royapettah, Chennai-600 014.
Corresponding Author: Saira Khatheeja
Hyperbilirubinemia is a condition of increased bilirubin in serum and analysis of this high bilirubin and other related components achieved in the serum by routine methods in clinical biochemistry and due to certain limitations. Current modalities of diagnosis have limitations such as poor sensitivity, specificity, reproducibility but expensive. Diagnostic methods should have greater sensitivity and also facilitate early detection of diseases to provide better prognosis. Further this FTIR-ATR spectroscopic method aids in analyzing multiple parameters / component in single spectral analysis which could be the added advantages to determine the other specified components over other existing methods.
In the present work, FTIR- ATR spectroscopic techniques will be employed to evaluate biomarkers by studying the variations on bio molecule composition in blood serum of control and experimental animals. Experiments were conducted on Phenylhydrazine induced hyperbilirubinemia wistar rat and biochemical and FTIR-ATR spectral analysis were done to rule out the clinical condition. The FTIR-ATR spectral analysis for hyperbilirubinemia were compared with other routine biochemical components assay followed by statistical methods.
Findings on induction of hyperbilirubinemia viz. Biochemical studies as well as FTIR-ATR spectral approach clearly establishes the effectiveness of the development of hyperbilirubinemia compared with control group of experimental wistar rat. The study on biochemical composition evaluation by spectroscopic techniques can be used not only for understanding the biological nature of the disease, but also for the diagnosis of the disease. Spectroscopy is the powerful tool for identifying types of chemical bonds /functional groups to identify the components of the sample qualitatively and quantitatively. The biochemical evaluations obtained with routine existing methods are comparable with the results of FTIR-ATR spectral analysis to diagnose the hyperbilirubinemia conditions in experimental wistar rat.
FTIR - ATR spectroscopic imaging has significant advantages composed to many other imaging methods for the characterization of biomolecules because it relies on the characteristic absorbance of corresponding molecular vibration in the sample functional group of chemical compounds such as carbohydrates , ester, albumin, proteins, nucleic acid well as inter atom chemical bonds. The ability to diagnose the early onset of disease, rapidly, non- invasively and unequivocally has multiple benefits. Quantitative estimation of Total Bilirubin in serum/ by FTIR –ATR spectrophotometric method is additional and useful in the diagnosis of hyperbilirubinemia due to any cause and is an indicator of liver functions.
Key words: hyperbilirubinemia, FTIR –ATR spectral analysis phenylhydrazine, induction studies, wistar rat, biochemical analysis.