International Journal of Health Sciences and Research

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Original Research Article

Year: 2018 | Month: December | Volume: 8 | Issue: 12 | Pages: 44-49

Isolation and Antibiotic Sensitivity Pattern of Salmonella Enterica Isolates from Livestock and Poultry of Haryana

Vipin Khasa1, Pamela Singh1, Nand Kishore Mahajan2

1Department of Biotechnology, Deen Bandhu Chhottu Ram University of Science and Technology, Murthal, Sonipat, Haryana
2Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana

Corresponding Author: Vipin Khasa


Salmonella enterica is one of the main enteric pathogen of animals and humans, which leads to diarrhea and mortality. Livestock and poultry are not only the main economic activity of farmers of Haryana state but also contribute food security to the state and country as a whole. Study on disease caused by Salmonellosis in livestock and poultry of Haryana was performed along with detail of serotypes circulating and antibiotic resistance profile of isolates during a period of three years. In present study, a total of one-hundred and twenty-five samples of bovine and poultry were attempted for isolation on selective media, followed by serotyping and antibiotic sensitivity testing by disc diffusion method. Thirty-two isolates out of a total of eighty-one samples collected from commercial broiler farms, were found positive for Salmonella spp., out of which S. typhimurium was found predominant (26/32) followed by rarely reported S. miami (4/32) and S. infantis (2/32). Out of forty-four fecal samples collected from cattle and buffaloes, no sample was found positive for Salmonellosis in present study. Antimicrobials Norfloxacin, Kanamycin, Amikacin, Chloramphenicol and Streptomycin were found most effective against Salmonella enterica isolates. Resistance of Salmonella enterica isolates was found highest towards Sulfa drugs, Tetracycline, Nitrofurantoin, Nalidixic acid and Ciprofloxacin. Detection of Salmonella enterica serovars from Haryana state confirms its occurrence but with changing pattern of circulating serotypes, more of public health importance and observation of multiple drug resistance necessitates routine testing of Salmonella spp. in poultry, better food safety and judicious use of antimicrobials in animals.

Key words: Salmonella, Broiler, Serotypes, Antibiotics, Haryana

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