Original Research Article
Year: 2017 | Month: September | Volume: 7 | Issue: 9 | Pages: 249-254
Human Milk’s Microbial Population Behavior In Vivo and Ex Vivo
Hrachya G. Hovhannisyan, Lusine V. Danielyan
SPC “Armbiotechnology” of the National Academy of Sciences, Yerevan, Armenia
Corresponding Author: Lusine V. Danielyan
ABSTRACT
The mobility of human milk microbial community the Armenian women has been studied in vivo and ex vivo. Human milk temporary microbes’ quantity was varied within stages of lactation. Culture based investigations revealed the greater microbial amount in colostrum and transitory milk means 105 CFU/ml, then it gradually reduced and reached 102 in mature milk. As the predominant microbes are able to ferment cow’s milk, it means that they belong to the genus of lactic acid bacteria. The size of the microbial population varied depending on the frequency of feeding, with a delay in feeding to 12 hours, it became about 1 log higher than the mean maximum. Examination of residential microbes’ growth character in different intact milk samples ex vivo at 37ºC in under anaerobic conditions have conditions revealed that the composition of human milk selectively promotes the growth of lactic acid bacteria whose titer can reach up to 1011 CFU / ml. The development of lactic acid bacteria successfully fermenting cow's milk does not cause visible coagulation of human milk, possibly due to the low concentration of casein proteins and the presence of anticoagulants. Since, probiotic bacteria thrive better in human milk than others; they will more effectively colonize breast feed infants intestine.
Key words: Human milk microbiota, lactic acid bacteria, in vivo, ex vivo