Original Research Article
Year: 2017 | Month: March | Volume: 7 | Issue: 3 | Pages: 100-106
Role of Tumor Necrosis Factor Alpha α and Interleukin 10 Gene Polymorphisms in Irritable Bowel Syndrome Patients
Eltayeb LB1, Aljafari AS2, Algazy SA3, AwatifSalih4, Yasseen Ali Yaseen5, Hisham Ali Waggiallah6
1Parasitology Dep. Faculty of Medical Laboratory Sciences; Omdurman Islamic University. Sudan
2Pathology department, College of Medicine-, Al-Jouf University. Saudia Arabia
3Parasitology Dep; Faculty of Medical Laboratory Sciences; Al-Neelain University. Sudan
4Al-neelaininstitute for Medical Research, Al-neelainUniversity. Sudan
5Department of Genaticsm Osmania University, India
6Clinical laboratory Dept. AlGhad International Colleges for Medical Sciences, Alriyadh. Saudiaarabia
Corresponding Author: Hisham Ali Waggiallah
ABSTRACT
Aims: The current study conducted to determine the frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) polymorphisms in subjects with IBS in Sudan.
Methods: After giving written consent, a total of 71 patients with symptoms of IBS according to the Rome III criteria and 40 controls were enrolled. DNA was extracted from peripheral blood leucocytes of subjects using salting out method. Polymorphisms were determined by PCR-RFLP method. SPSS software program was used for statistical analysis.
Results: Patients with irritable bowel syndrome had significantly reduced frequencies of the high producer genotype for interleukin 10 than controls (21% v 32%; p=0.003). there was no significant difference in the frequency of the genotypes or the alleles of the IL-10 (-1082G/A) and the TNF-α (-308G/A) polymorphisms between IBS and the controls, however it was demonstrated that the high producer (AA) TNF α genotype was more prevalent in IBS patients compared to healthy controls (8.5% VS zero). IBS patients were classified as IBS-D (53.5 %), IBS-C (18.3 %) and IBS-A/M (28.2 %) and there were no statistical differences IBS subgroups, were found among these IBS subgroups 57% of patients had family history of IBS.
Conclusions: The frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) genotypes was similar in IBS and controls. However, there was a greater frequency of the low producer of IL-10 in those subjects with IBS-D, suggesting a genetic predisposition to abnormal immune regulation due to a lower anti-inflammatory cytokines IL10 in this subgroup.
Key words: IL-10, TNF α,IBS, polymorphism.