Original Research Article
Year: 2017 | Month: January | Volume: 7 | Issue: 1 | Pages: 68-74
Asymptomatic Bacteriuria due to MDR Organisms in Type 2 Diabetes Mellitus Patients
Ketaki Vyankatesh Kulkarni1, Niranjan P. Pathak2
1Assistant Professor, Dept. Of Microbiology, MIMER Medical College, Talegaon-Dabhade, Pune, India. 410507.
2Assistant Professor, Dept. Of General Medicine, MIMER Medical College, Talegaon-Dabhade, Pune, India. 410507.
Corresponding Author: Ketaki Vyankatesh Kulkarni
ABSTRACT
Background & Objectives: Diabetic subjects, especially women, show high prevalence of asymptomatic bacteriuria (ASB). The aetiology and the antibiotic resistance of uropathogens have been changing over the past years. The present study was undertaken to evaluate the prevalence of asymptomatic bacteriuria & occurrence of multidrug resistant (MDR) strains of bacteria in Type 2 Diabetes Mellitus patients.
Methods: 200 type 2 diabetic males and females (aged between 30-80 years) who attended Maharashtra Institute of Medical Education & Research & BSTR hospital, Pune, India were included in the study. Mid-stream urine samples were collected from patients aseptically into sterile wide mouth container examined microscopically & was cultured using standard techniques. Isolates were tested against separate antibiotics for gram negative and gram positive organisms by the disc diffusion method.
Results: Significant bacteriuria was observed in 52% of urine samples (50 females and 54 males). Bacteria isolated included Escherichia coli (31%), Klebsiella pneumoniae (13%), Citrobacter koseri(13%), Pseudomonas aeruginosa (10%), Proteus vulgaris (3%), Acinetobacter baumannii (2%) among GNB & Staphylococcus aureus (25%) among GPCs.MDR was observed in 67 (60.36%) of the total 111 isolates obtained. Highest occurrence of MDR was observed among Acinetobacter baumannii (100%) followed by Pseudomonas aeruginosa (80%).
Conclusion: The high prevalence of ASB and the multiple resistances of most isolates is a major concern which suggests the need to enhance sensitization against antibiotic abuse so as to curb the spread of multi resistant uropathogens in the study area.
Key words: Asymptomatic Bacteriuria, Escherichia coli, MDR, Midstream urine, Type 2 diabetes mellitus.