Year: 2024 | Month: December | Volume: 14 | Issue: 12 | Pages: 180-190
DOI: https://doi.org/10.52403/ijhsr.20241221
Antibiogram and Bacterial Profile Analysis of Various Clinical Samples at Tertiary Healthcare Centre in North-West region of Punjab
Rupinder Bakshi1, Karashdeep Kaur2, Palika Sharma2, Jaspreet Kaur Boparai2, Satinder Kaur2
1Professor and Head, Department of Microbiology, Government Medical College, Patiala-147001, IND,
2Research Scientist, Viral Research & Diagnostic Laboratory, Department of Microbiology, Government Medical College, Patiala-147001, IND
Corresponding Author: Dr. Rupinder Bakshi
ABSTRACT
Background: Antimicrobial resistance is a significant barrier in achieving the sustainable development and is one of the leading global public health menaces to humanity. Present study determined the antibiogram profiles of bacteria isolated from different body site infections among patients admitted to tertiary care Hospital, Punjab, Northwest India.
Methods: A cross-sectional study was carried out using 11201 clinical samples at the tertiary care hospital Patiala from June 2023 to June 2024. Following the criteria of the Clinical and Laboratory Standards Institute S.aureus isolates and further tested for antimicrobial activity using standard procedures
Results: From the 11201 clinical specimens processed, a total of 1638 urine, 403 blood and 2126 other body fluids (pus and sputum) were culture positive for bacterial pathogens. The prevalence of infection was highest among middle aged males in blood and other fluids where middle aged female’s predominance was found in urine infection. In urine samples, Escherichia coli (63.67%) from Gram-negative and Enterococcus (6.53%) and S. Aureus (4.45%) from gram positive were the predominant bacterial. Blood sample showed the prevalence of K. pneumonia (28.28%)amonggram negative and Enterococcus (27.79%) among gram positive. K. pneumonia (20.93%) and S. Aureus (29.25%) were predominant in other fluid infections. antimicrobial resistance pattern in all clinical samples and results suggest that most of the bacteria isolated from studied clinical samples are less susceptible ranging from 0% to 35% mostly towards penicillin, norfloxacin, Cefuroxime and ampicillin. Intermediate susceptibility ranged from 40%-85% towards amikacin, Gentamicin and Meropenem. High susceptibility varied according to bacteria and sample type and it ranged between 90%-100%. In urine sample high susceptibility was observed for P. aeruginosa (100%) and A. Baumanni (100%). In Blood samples S. aureus showed the highest susceptibility towards amikacin (100%). Many antibiotics showed 100% susceptibility in other body fluids infections such as P. aeruginosa towards Norfloxacin, S. aureus towards amoxicillin, enetrococcus towards ampicillin, gentamicin and amikacin.
Conclusion: Overall result of the present investigation showed that penicillins are least susceptible and amikacin is highly susceptible antibiotic against the studied infections. By focusing on locally prevalent antibiotic resistance patterns instead of regionally and internationally available data on antibiogram, we may ensure faster patient recovery at lower costs and avoid the unwarranted development of drug resistance.
Key words: Antibiogram, Antibiotic resistance, Bacterial isolates, clinical specimen, Punja